Participation of CD45, NKR-P1A and ANK61 antigen in rat hepatic NK cell (pit cell)mediated target cell cytotoxicity

• Published in: World journal of gastroenterology : WJG Vol. 6; no. 4; pp. 546 - 552
• Main Authors: Luo, Dian-Zhong, Vermijlen, David, Ahishali, B lent, Triantis, Vasilis, Vanderkerken, Karin, Kuppen, Peter JK Kuppen, Wisse, Eddie
• Format: Journal Article
• Language: English
• Published: United States Laboratory for Cell Biology and Histology 01-08-2000; K Vanderkerken is a postdoctoral researcher of the Fund for Scientific Research Flanders.%Department of Surgery and Pathology, Leiden University Medical Center, Leiden , The Netherlands; Department of Pathology, Guangxi Medical University, Nanning, China%Laboratory for Cell Biology and Histology.%Department of Hematology and Immunology, Free University of Brussels VUB, Brussels-Jette, Belgium; Baishideng Publishing Group Inc
• Subjects: Original; hepatic NK cells; pit cells; apoptosis; perforin/granzyme pathway; cytolysis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v6.i4.546

AIM:Several triggering receptors have been described to be involved in natural killer (NK) cell-mediated target cytotoxicity. In these studies, NK cells deriv ed from blood or spleen were used. Pit cells are liver-specific NK cells that possess a higher level of natural cytotoxicity and a different morphology when compared to blood NK cells. The aim of this study was to characterize the role of the NK triggering molecules NKR P1A, ANK61 antigen, and CD45 in pit cell medi ated killing of target cells.METHODS:( 51) Crrelease and DNA fragmentation were used to quantify target cell lysis and apoptosis, respectively.RESULTS:Flow cytometric analysis showed that pit cells expressed CD45, NK R P1A, and ANK61 antigen. Treatment of pit cells with monoclonal antibody (mAb)to CD45 (ANK74) not only inhibited CC531s or YAC 1 target lysis but also apopt osis induced by pit cells. The mAbs to NKR P1A (3.2.3) and ANK61 antigen (ANK61 )had no effect on pit cell mediated CC531s or YAC 1 target cytolysis or apopto sis, while they did increase the Fcgamma receptor positive (FcgammaR(+)) P815 cytolysi s and apoptosis. This enhanced cytotoxicity could be inhibited by 3,4 dichloroi socoumarin, an inhibitor of granzymes.CONCLUSION:These results indicate that CD45 participates in pit cell med iated CC531s and YAC-1 target cytolysis and apoptosis. NKR-P1A and ANK61 antigen on pit cells function as activation structures against Fc gammaR( +) P 815 cells, which was mediated by the perforin/granzyme pathway.