Segregation of saturated chain lipids in pulmonary surfactant films and bilayers

• Published in: Biophysical journal Vol. 82; no. 4; pp. 2041 - 2051
• Main Authors: Nag, Kaushik, Pao, Jin-Si, Harbottle, Robert R, Possmayer, Fred, Petersen, Nils O, Bagatolli, Luis A
• Format: Journal Article
• Language: English
• Published: United States Biophysical Society 01-04-2002
• Subjects: 2-Naphthylamine - analogs & derivatives; 2-Naphthylamine - chemistry; Animals; Biophysics; Calorimetry, Differential Scanning; Cattle; Chloroform - chemistry; Fluorescent Dyes - chemistry; Laurates - chemistry; Lipid Bilayers - chemistry; Lipids; Lipids - chemistry; Lungs; Models, Biological; Photons; Proteins; Pulmonary Alveoli - metabolism; Temperature
• ISSN: 0006-3495; 1542-0086
• DOI: 10.1016/S0006-3495(02)75552-5

The physical properties of organized system (bilayers and monolayers at the air water interface) composed of bovine lipid extract surfactant (BLES) were studied using correlated experimental techniques. 6-Dodecanoyl-2-dimethylamino-naphthalene (LAURDAN)-labeled giant unilamelar vesicles (mean diameter approximately 30 microm) composed of BLES were observed at different temperatures using two-photon fluorescence microscopy. As the temperature was decreased, dark domains (gel-like) appeared at physiological temperature (37 degrees C) on the surface of BLES giant unilamelar vesicles. The LAURDAN two-photon fluorescent images show that the gel-like domains span the lipid bilayer. Quantitative analysis of the LAURDAN generalized polarization function suggests the presence of a gel/fluid phase coexistence between 37 degrees C to 20 degrees C with low compositional and energetic differences between the coexisting phases. Interestingly, the microscopic scenario of the phase coexistence observed below 20 degrees C shows different domain's shape compared with that observed between 37 degrees C to 20 degrees C, suggesting the coexistence of two ordered but differently organized lipid phases on the bilayer. Epifluorescence microscopy studies of BLES monomolecular films doped with small amounts of fluorescent lipids showed the appearance and growth of dark domains (liquid condensed) dispersed in a fluorescent phase (liquid expanded) with shapes and sizes similar to those observed in BLES giant unilamelar vesicles. Our study suggests that bovine surfactant lipids can organize into discrete phases in monolayers or bilayers with equivalent temperature dependencies and may occur at physiological temperatures and surface pressures equivalent to those at the lung interface.