Identification of phenotypic markers of B cells from patients with Chagas disease

• Published in: Parasite immunology Vol. 35; no. 7-8; pp. 214 - 223
• Main Authors: Fares, R. C. G., Correa‐Oliveira, R., Araújo, F. F., Keesen, T. S. L., Chaves, A. T., Fiuza, J. A., Ferreira, K. S., Rocha, M. O. C., Gomes, J. A. S.
• Format: Journal Article
• Language: English
• Published: England 01-07-2013
• Subjects: Adult; Antigens, CD - analysis; B lymphocytes; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Biomarkers - analysis; Caspase 3 - metabolism; Chagas disease; Chagas Disease - immunology; Chagas Disease - metabolism; Female; HLA-DR Antigens - immunology; HLA-DR Antigens - metabolism; Humans; IL‐10; Immunophenotyping; immunoregulation; Interleukin-10 - biosynthesis; Interleukin-10 - immunology; Male; Middle Aged; phenotypic markers; Transforming Growth Factor beta - metabolism; Trypanosoma cruzi - immunology; Chagas disease; immunoregulation; phenotypic markers; B lymphocytes; IL-10
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12038

Summary
Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of B lymphocytes. Patients with Chagas disease have shown increased percentage of B cells producing IL‐10. However, there are no reports of the phenotypic markers of B cells producing IL‐10 in patients with Chagas disease. For the first time in the literature, we evaluated the phenotypic profile of distinct markers of B cells from peripheral blood of noninfected individuals and patients with Chagas disease. Our results showed that patients with Chagas disease had a higher expression of CD21 and CD24 on the surface of CD19+ B cells, while CD43 and CD23 were expressed equally in all groups. Moreover, the expression of MHC‐II (HLA‐DR), CD80, CD86, caspase‐3, granzyme B and intracellular IL‐10 and TGF‐β by CD19+ B cells was higher in patients with Chagas disease. The results of IL‐10 production within CD19+CD5+CD1d+ B cells showed a higher percentage of this cytokine in patients with Chagas disease. Thus, our data bring a new knowledge about distinct markers of B cells in immune responses of Chagas disease.