Signatures of selection identify loci associated with fat tail in sheep

• Published in: Journal of animal science Vol. 93; no. 10; pp. 4660 - 4669
• Main Authors: Moioli, B, Pilla, F, Ciani, E
• Format: Journal Article
• Language: English
• Published: United States 01-10-2015
• Subjects: Animals; Body Fat Distribution - veterinary; Breeding; Gene Frequency; Genome; Genotype; Polymorphism, Single Nucleotide; Sheep - genetics; Tail - physiology
• ISSN: 1525-3163
• DOI: 10.2527/jas.2015-9389

Identification of genomic regions that have been targets of selection for phenotypic traits is one of the most challenging applications of dense marker panels in animal genetics. In this study, a genomewide scan using approximately 50,000 SNP was performed in an attempt to identify genomic regions associated with fat deposition in sheep, the importance of this not only being limited to livestock facing future climate changes but also for elucidating the physiology of lipid metabolism. The genotyping results obtained with the Ovine SNP50K BeadChip in 2 fat tail breeds were compared with those obtained in 13 thin tail breeds. Direct sequencing of the annotated genes located in proximity to the markers with opposite allele frequency in thin tail vs. fat tail sheep gave additional SNP of interest. To further confirm the results of the genomewide scan, we genotyped the SNP within these genes in the 2 groups of sheep. A missense mutation in the gene, with different allele frequency in the 2 groups, was detected. The results indicated and as the most probable genes involved in the fat tail phenotype.