miRNA expression profile of mucoepidermoid carcinoma

• Published in: Oral diseases Vol. 24; no. 4; pp. 537 - 543
• Main Authors: Binmadi, NO, Basile, JR, Perez, P, Gallo, A., Tandon, M, Elias, W, Jang, SI, Alevizos, I
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01-05-2018
• Subjects: Cancer; Dentistry; Glands; Malignancy; miRNA; miR‐302; mucoepidermoid carcinoma; Oral cancer; Salivary gland tumours; Tumor cell lines; Tumors; miRNA; miR-302; Salivary gland tumours; mucoepidermoid carcinoma
• ISSN: 1354-523X; 1601-0825
• DOI: 10.1111/odi.12800

Objectives
MicroRNAs (miRNAs) are single‐stranded RNAs that have been implicated in cancer initiation and progression and act as tumour suppressors or oncogenes. In this study, miRNA profiling was conducted on the most frequent malignancy of salivary glands, mucoepidermoid carcinoma (MEC), in comparison with normal tissues.
Materials and Methods
The TaqMan Human miRNA Cards Array was used for the miRNA profiling of MEC and normal tissues. To validate the differentially expressed miRNAs in MEC, we used real‐time PCR (qRT‐PCR).
Results
miR‐302a was the most significantly increased miRNA in cancer tissues (p < .05). Here, we demonstrate that the upregulation of miR‐302a expression in SGT cell lines induced cancer cell invasion in vitro.
Conclusions
These promising results suggest the need for further studies to establish mir‐302a as a marker of invasion and aggressiveness in MEC.