Quantitative PCR and Immunohistochemical Analyses of HMGB1 and RAGE Expression in Canine Disseminated Histiocytic Sarcoma (Malignant Histiocytosis)
Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high...
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| Published in: | Anticancer research Vol. 31; no. 5; pp. 1541 - 1548 |
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International Institute of Anticancer Research
01-05-2011
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| Abstract | Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high mobility group box1 protein (HMGB1) have been shown to be required for the maturation and migration of DCs. Thus, deregulation of the expression of these genes could have a major effect on the progression of histiocytic disorders.
Neoplastic canine DHS samples and non-neoplastic control samples were analysed immunohistochemically and via real-time PCR.
Significant down-regulation of RAGE in the lung tumour samples and down-regulation of HMGB1 in the lung, lymph node and spleen tumour samples were detected compared to their non-neoplastic counterparts.
RAGE and HMGB1 expression down-regulation in canine DHS points to a role in the progression of histiocytic disorders. |
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| AbstractList | BACKGROUNDDisorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high mobility group box1 protein (HMGB1) have been shown to be required for the maturation and migration of DCs. Thus, deregulation of the expression of these genes could have a major effect on the progression of histiocytic disorders.MATERIALS AND METHODSNeoplastic canine DHS samples and non-neoplastic control samples were analysed immunohistochemically and via real-time PCR.RESULTSSignificant down-regulation of RAGE in the lung tumour samples and down-regulation of HMGB1 in the lung, lymph node and spleen tumour samples were detected compared to their non-neoplastic counterparts.CONCLUSIONRAGE and HMGB1 expression down-regulation in canine DHS points to a role in the progression of histiocytic disorders. Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high mobility group box1 protein (HMGB1) have been shown to be required for the maturation and migration of DCs. Thus, deregulation of the expression of these genes could have a major effect on the progression of histiocytic disorders. Neoplastic canine DHS samples and non-neoplastic control samples were analysed immunohistochemically and via real-time PCR. Significant down-regulation of RAGE in the lung tumour samples and down-regulation of HMGB1 in the lung, lymph node and spleen tumour samples were detected compared to their non-neoplastic counterparts. RAGE and HMGB1 expression down-regulation in canine DHS points to a role in the progression of histiocytic disorders. Background: Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high mobility group box1 protein (HMGB1) have been shown to be required for the maturation and migration of DCs. Thus, deregulation of the expression of these genes could have a major effect on the progression of histiocytic disorders. Materials and Methods: Neoplastic canine DHS samples and non-neoplastic control samples were analysed immunohistochemically and via realtime PCR. Results: Significant down-regulation of RAGE in the lung tumour samples and down-regulation of HMGB1 in the lung, lymph node and spleen tumour samples were detected compared to their non-neoplastic counterparts. Conclusion: RAGE and HMGB1 expression down-regulation in canine DHS points to a role in the progression of histiocytic disorders. |
| Author | WEFSTAEDT, Patrick EBERLE, Nina MURUA ESCOBAR, Hugo NOLTE, Ingo KLEINSCHMIDT, Sven HEWICKER-TRAUTWEIN, Marion STERENCZAK, Katharina A BULLERDIEK, Jorn |
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| Keywords | Immunohistochemistry disseminated histiocytic sarcoma Histiocytic lymphoma HMGB 1 Cancerology Receptor for advanced glycation endproducts Lymphoproliferative syndrome Disseminated Dog Quantitative analysis Fissipedia Carnivora HMGB1 Protein Malignant histiocytosis Malignant hemopathy RAGE Non Hodgkin lymphoma Quantitative PCR Polymerase chain reaction Anatomic pathology Vertebrata Mammalia Animal Molecular biology Cancer |
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| Snippet | Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as... BACKGROUNDDisorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known... Background: Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known... |
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| SubjectTerms | Animals Biological and medical sciences Dogs Down-Regulation Female Gene Expression Regulation, Neoplastic Hematologic and hematopoietic diseases Histiocytic Sarcoma - genetics Histiocytic Sarcoma - metabolism Histiocytic Sarcoma - pathology HMGB1 Protein - genetics HMGB1 Protein - metabolism Immunoenzyme Techniques Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Receptor for Advanced Glycation End Products Receptors, Immunologic - genetics Receptors, Immunologic - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Tumors |
| Title | Quantitative PCR and Immunohistochemical Analyses of HMGB1 and RAGE Expression in Canine Disseminated Histiocytic Sarcoma (Malignant Histiocytosis) |
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