Quantitative PCR and Immunohistochemical Analyses of HMGB1 and RAGE Expression in Canine Disseminated Histiocytic Sarcoma (Malignant Histiocytosis)

• Published in: Anticancer research Vol. 31; no. 5; pp. 1541 - 1548
• Main Authors: STERENCZAK, Katharina A, KLEINSCHMIDT, Sven, WEFSTAEDT, Patrick, EBERLE, Nina, HEWICKER-TRAUTWEIN, Marion, BULLERDIEK, Jorn, NOLTE, Ingo, MURUA ESCOBAR, Hugo
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-05-2011
• Subjects: Animals; Biological and medical sciences; Dogs; Down-Regulation; Female; Gene Expression Regulation, Neoplastic; Hematologic and hematopoietic diseases; Histiocytic Sarcoma - genetics; Histiocytic Sarcoma - metabolism; Histiocytic Sarcoma - pathology; HMGB1 Protein - genetics; HMGB1 Protein - metabolism; Immunoenzyme Techniques; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Male; Medical sciences; Receptor for Advanced Glycation End Products; Receptors, Immunologic - genetics; Receptors, Immunologic - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Tumors; Immunohistochemistry; disseminated histiocytic sarcoma; Histiocytic lymphoma; HMGB 1; Cancerology; Receptor for advanced glycation endproducts; Lymphoproliferative syndrome; Disseminated; Dog; Quantitative analysis; Fissipedia; Carnivora; HMGB1 Protein; Malignant histiocytosis; Malignant hemopathy; RAGE; Non Hodgkin lymphoma; Quantitative PCR; Polymerase chain reaction; Anatomic pathology; Vertebrata; Mammalia; Animal; Molecular biology; Cancer
• ISSN: 0250-7005; 1791-7530

Disorders of histiocytic origin affecting humans and dogs share various similarities. Canine disseminated histiocytic sarcoma (DHS) (formerly known as malignant histiocytosis) is an aggressive neoplasm of interstitial dendritic cells (DCs). The receptor for glycation end products (RAGE) and the high mobility group box1 protein (HMGB1) have been shown to be required for the maturation and migration of DCs. Thus, deregulation of the expression of these genes could have a major effect on the progression of histiocytic disorders.
Neoplastic canine DHS samples and non-neoplastic control samples were analysed immunohistochemically and via real-time PCR.
Significant down-regulation of RAGE in the lung tumour samples and down-regulation of HMGB1 in the lung, lymph node and spleen tumour samples were detected compared to their non-neoplastic counterparts.
RAGE and HMGB1 expression down-regulation in canine DHS points to a role in the progression of histiocytic disorders.