Expression of Transketolase-like 1 Protein (TKTL1) in Human Endometrial Cancer

• Published in: Anticancer research Vol. 30; no. 5; pp. 1653 - 1659
• Main Authors: KROCKENBERGER, M, ENGEL, J. B, SCHMIDT, M, KOHRENHAGEN, N, HÄUSLER, S. F. M, DOMBROWSKI, Y, KAPP, M, DIETL, J, HONIG, A
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01-05-2010
• Subjects: Aged; Biological and medical sciences; Cell Line, Tumor; Endometrial Neoplasms - metabolism; Female; Female genital diseases; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Glucose - metabolism; Glucose Transporter Type 1 - metabolism; Glycolysis; Gynecology. Andrology. Obstetrics; Humans; Immunohistochemistry - methods; Medical sciences; Middle Aged; Oxygen - chemistry; RNA, Messenger - metabolism; Transketolase - biosynthesis; Tumors; Human; GLUT1; Endometrium cancer; Enzyme; Transferases; Transketolase; pAkt; Glucose; Malignant tumor; Metabolism; Transketolase-like-1 protein (TKTL1); Protein; Female genital diseases; Cancerology; GLUT1 glucose transporter; Uterine diseases; Glycolysis; endometrial cancer; aerobic glycolysis; glucose metabolism; Cancer
• ISSN: 0250-7005; 1791-7530

Malignant tumors metabolize glucose to lactate even in the presence of oxygen (aerobic glycolysis). The metabolic switch from oxidative glycolysis to non-oxidative fermentation of glucose and proteins performed by the tumor cells seems to be associated with TKTL1 and pAkt overexpression. Therefore the aim of the present study was to investigate the expression of TKTL1 and pAkt in human specimens of endometrial cancer as compared to benign endometrium. Additionally, expression of the glucose transporter GLUT1 was also investigated as aerobic glycolysis is associated with an increased need for glucose.
Levels of TKTL1, pAkt, and GLUT1 expression were immunhistochemically evaluated on paraffin embedded biopsy material from 10 benign and 41 malignant endometrial tissue samples. TKTL1 mRNA levels in the endometrial cancer cell lines Ishikawa and HEC-1A were evaluated by RT-PCR.
Expression of TKTL1, GLUT1 and pAKT was significantly increased in endometrial carcinomas as compared to benign endometrial tissue. There was a significantly weaker TKTL1 expression in highly differentiated G1 tumors. In the human endometrial cancer cell lines Ishikawa and HEC-1A, TKTL1 mRNA was clearly detectable.
The levels of TKTL1, GLUT1 and pAKT expression point to the glycolytic phenotype of malignant endometrial tissue. Given the pronounced TKTL1 expression across all different subtypes of endometrial cancer, this protein could serve as a target for future cancer treatments.