Adjuvant effect of dendritic cells transduced with recombinant vaccinia virus expressing HPV16-E7 is inhibited by co-expression of IL12

• Published in: International journal of oncology Vol. 24; no. 6; p. 1581
• Main Authors: Mackova, Jana, Kutinova, Luda, Hainz, Petr, Krystofova, Jitka, Sroller, Vojtech, Otahal, Pavel, Gabriel, Pavel, Nemeckova, Sarka
• Format: Journal Article
• Language: English
• Published: Greece 01-06-2004
• Subjects: Adjuvants, Immunologic; Animals; Antigen Presentation; Cancer Vaccines - immunology; Cancer Vaccines - therapeutic use; Dendritic Cells - immunology; Female; Genetic Therapy; Genetic Vectors; Humans; Immunization; Immunotherapy; Interleukin-12 - genetics; Interleukin-12 - immunology; Interleukin-12 - therapeutic use; Mice; Mice, Inbred C57BL; Neoplasms, Experimental - immunology; Neoplasms, Experimental - therapy; Neoplasms, Experimental - virology; Oncogene Proteins, Viral - genetics; Oncogene Proteins, Viral - immunology; Oncogene Proteins, Viral - therapeutic use; Papillomaviridae - physiology; Papillomavirus E7 Proteins; Papillomavirus Infections - genetics; Papillomavirus Infections - immunology; Papillomavirus Infections - therapy; Survival Rate; Th1 Cells - immunology; Transduction, Genetic; Vaccinia virus - genetics
• ISSN: 1019-6439

Dendritic cells (DC) enhanced the immunogenicity of recombinant vaccinia viruses (rVV) expressing the E7 protein of HPV16, a tumor-associated antigen (TAA). Immunization with DC transduced by rVV generated from strain Praha or MVA induced better protection against the growth of transplanted TC-1 tumors in C57Bl/6 mice than did immunization with either of these rVVs administered alone by the same route. Interestingly, DC transduced with a double recombinant vaccinia virus expressing E7 protein together with the Th1-polarizing cytokine IL12, which has been shown to enhance the cellular response in several other systems, induced lower anti-tumor immunity than DC transduced with rVV expressing E7 protein alone. The inhibitory effect mediated by IL12 on immunization with rVV-infected DC was dose-dependent and was observed after immunization with DC transduced with IL12-expressing rVV even at low multiplicity.