The use of DNase for preparation of host tissue DNA-free rickettsial suspensions

Treatment of Rickettsia prowazekii (strain Breinl) grown in chick embryo (CE) yolk sacs with DNase resulted in preparation of host tissue DNA-free rickettsial suspensions suitable for isolation of rickettsial DNA. Of two DNA fractions obtained, the first and main one corresponded to rickettsial DNA...

Full description

Saved in:

Bibliographic Details
Published in:	Acta virologica (Anglickâa verze) Vol. 28; no. 1; p. 73
Main Authors:	Ogarkova, O A, Balayeva, N M
Format:	Journal Article
Language:	English
Published:	Slovakia 01-01-1984
Subjects:	Deoxyribonucleases DNA, Bacterial - isolation & purification Rickettsia - isolation & purification Spectrophotometry, Ultraviolet Yolk Sac - microbiology
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Treatment of Rickettsia prowazekii (strain Breinl) grown in chick embryo (CE) yolk sacs with DNase resulted in preparation of host tissue DNA-free rickettsial suspensions suitable for isolation of rickettsial DNA. Of two DNA fractions obtained, the first and main one corresponded to rickettsial DNA (29% G + C) based on the physico-chemical characteristics, whereas the nature of second supplementary fraction (47.5% G + C) was not determined.
ISSN:	0001-723X