Hydrogen peroxide-induced apoptosis in human gingival fibroblasts

• Published in: International journal of clinical and experimental pathology Vol. 8; no. 12; pp. 15563 - 15572
• Main Authors: Gutiérrez-Venegas, Gloria, Guadarrama-Solís, Adriana, Muñoz-Seca, Carmen, Arreguín-Cano, Juan Antonio
• Format: Journal Article
• Language: English
• Published: United States e-Century Publishing Corporation 01-01-2015
• Subjects: Adolescent; Apoptosis - drug effects; Apoptosis Regulatory Proteins - metabolism; Cells, Cultured; Dose-Response Relationship, Drug; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Gingiva - drug effects; Gingiva - metabolism; Gingiva - pathology; Humans; Hydrogen Peroxide - toxicity; Mitochondria - drug effects; Mitochondria - metabolism; Mitochondria - pathology; Original; Oxidative Stress - drug effects; Time Factors; Tooth Bleaching Agents - toxicity; Young Adult; discolored teeth; Human gingival fibroblasts; apoptosis; hydrogen peroxide
• ISSN: 1936-2625

In the process of bleaching vital, discolored teeth, low concentrations of hydrogen peroxide (H2O2) are effective alternatives to heat-activated 30% H2O2. However, interest has been expressed in the assessment of pathological effects of long-term exposure to bleaching agents such as irritation and ulceration of the gingival or other soft tissues. The aim of the present study was to determine the effect of hydrogen peroxide on apoptosis in human gingival fibroblasts (HGF). Cytochrome c, Bcl-2, Bax, Bid and caspase-3 protein expression were detected by Western blotting. HGF cell apoptosis induced by H2O2 was both dose and time dependent. The addition of H2O2 resulted in the release of cytochrome c to the cytosol, and an increase of Caspase-3 cleavage. Data suggest that oxidative stress-induced apoptosis in HGF is intrinsic pathway involved the release of apoptotic signal from mitochondria.