Relationship between topoisomerase II levels and resistance to topoisomerase II inhibitors in lung cancer cell lines

Topoisomerase II is a key target of many anticancer drugs used to treat lung cancer. We measured the expression of topoisomerase II alpha and beta mRNA's and also the levels of cellular topoisomerase II alpha and beta protein and concluded that topoisomerase II alpha levels are important in cel...

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Bibliographic Details
Published in:	Anticancer research Vol. 16; no. 4A; p. 1603
Main Authors:	Houlbrook, S, Harris, A L, Carmichael, J, Stratford, I J
Format:	Journal Article
Language:	English
Published:	Greece 01-07-1996
Subjects:	Amsacrine - toxicity Antineoplastic Agents - toxicity Blotting, Western Cell Line Chlorambucil - toxicity DNA Topoisomerases, Type II - adverse effects DNA Topoisomerases, Type II - biosynthesis Doxorubicin - toxicity Drug Resistance, Neoplasm Enzyme Inhibitors - toxicity Etoposide - toxicity Humans Lung Neoplasms Mitoxantrone - toxicity RNA, Messenger - biosynthesis Transcription, Genetic - drug effects Tumor Cells, Cultured
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Summary:	Topoisomerase II is a key target of many anticancer drugs used to treat lung cancer. We measured the expression of topoisomerase II alpha and beta mRNA's and also the levels of cellular topoisomerase II alpha and beta protein and concluded that topoisomerase II alpha levels are important in cellular resistance to the topoisomerase II inhibitors examined. This can be clearly seen in pairs of matched cell lines. However, when looking at a panel of cell lines with a range of histological types the importance of the enzyme can be masked by other cellular characteristics such as repair and detoxification mechanisms.
ISSN:	0250-7005