Improved method for the concentration and purification of faecal cysts of Entamoeba histolytica for use as antigen

Human stool samples are the most widely used source of E. histolytica cysts for diagnosis and research. Cysts concentrated from faecal samples are contaminated with faecal debris and bacteria and are unsuitable for use as antigens. Other gradient purification and sedimentation procedures result in c...

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Bibliographic Details
Published in:	Journal of tropical medicine and hygiene Vol. 96; no. 4; p. 249
Main Authors:	Jyothi, R, Foerster, B, Hamelmann, C, Shetty, N P
Format:	Journal Article
Language:	English
Published:	England 01-08-1993
Subjects:	Animals Antigens, Protozoan - isolation & purification Centrifugation Centrifugation, Density Gradient Entamoeba histolytica - immunology Entamoeba histolytica - isolation & purification Entamoebiasis - parasitology Feces - parasitology Humans
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Summary:	Human stool samples are the most widely used source of E. histolytica cysts for diagnosis and research. Cysts concentrated from faecal samples are contaminated with faecal debris and bacteria and are unsuitable for use as antigens. Other gradient purification and sedimentation procedures result in considerable cyst losses. We present an effective and simple purification procedure using varying densities of Percoll that yields an adequate cyst concentration from a moderate cyst load in a single stool sample.
ISSN:	0022-5304