Lymphocytes in patients with variable immunodeficiency and panhypogammaglobulinemia. Evaluation of B and T cell surface markers and a proposed classification

• Published in: The Journal of clinical investigation Vol. 53; no. 3; pp. 834 - 840
• Main Authors: Dickler, H B, Adkinson, Jr, N F, Fisher, R I, Terry, W D
• Format: Journal Article
• Language: English
• Published: United States 01-03-1974
• Subjects: Adolescent; Adult; Agammaglobulinemia - blood; Agammaglobulinemia - classification; Agammaglobulinemia - immunology; B-Lymphocytes - immunology; Cell Membrane - immunology; Cell Wall; Female; Humans; Immunoglobulin G - analysis; Immunologic Deficiency Syndromes - blood; Immunologic Deficiency Syndromes - classification; Immunologic Deficiency Syndromes - immunology; Lymphocytes - immunology; Male; Methods; Middle Aged; Protein Binding; T-Lymphocytes - immunology
• ISSN: 0021-9738
• DOI: 10.1172/JCI107623

Peripheral blood lymphocytes from 15 patients with variable immunodeficiency and severe panhypogammaglobulinemia were evaluated for B and T cell surface markers. B cells were enumerated by immunofluorescent detection of both surface immunoglobulin (Ig) and the ability to bind aggregated Ig complexes. T cells were identified by their ability to form nonimmune rosettes with sheep red blood cells. Four distinct patterns were observed which were designated types I-IV. Type I: six patients had normal percentages (8.5-19.0%) of Ig-bearing B lymphocytes. Type II: four patients were observed to have B lymphocytes (4.5-15.0%) which lacked fluorescence-detectable surface Ig. Type III: the peripheral blood of these four patients contained a subpopulation (11.3-20.0%) of lymphocytes which apparently lacked both B and T cell markers ("null" cells). Type IV: one patient's blood was characterized by a subpopulation (18.0-22.0%) of lymphocytes which bore both B and T cell markers. Patients of each type had some clinical features in common. It is concluded that evaluation of lymphocyte surface markers provides a means of separating patients with variable immunodeficiency and panhypogammaglobulinemia into distinct groups which appear to differ in the nature of their fundamental defect.