Isolation and propagation of colon cancer stem cells

The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 1035; p. 247
Main Authors:	Prasetyanti, Pramudita R, Zimberlin, Cheryl, De Sousa E Melo, Felipe, Medema, Jan Paul
Format:	Journal Article
Language:	English
Published:	United States 01-01-2013
Subjects:	Adenoma - pathology Animals Cell Dedifferentiation Cell Differentiation Cell Line, Tumor Cell Proliferation Cell Separation Cells, Cultured Coculture Techniques Colonic Neoplasms - pathology Culture Media, Conditioned Feeder Cells Humans Mice Neoplastic Stem Cells - physiology
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Summary:	The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.
ISSN:	1940-6029
DOI:	10.1007/978-1-62703-508-8_21