Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation
To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation. Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines....
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| Published in: | Anticancer research Vol. 34; no. 9; pp. 4939 - 4948 |
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| Main Authors: | , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
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01-09-2014
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| Abstract | To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.
Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.
The major gene expression changes during irradiation are related to WNT signaling pathway. |
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| AbstractList | Background: To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation. Materials and Methods: Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray. Results: Four established cell (A549-2G, A5495G, H1650-2G and H1650-5G) were shown to be radioresistant (p[< or =] 0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines. Conclusion: The major gene expression changes during irradiation are related to WNT signaling pathway. BACKGROUNDTo identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.MATERIALS AND METHODSLung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.RESULTSFour established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.CONCLUSIONThe major gene expression changes during irradiation are related to WNT signaling pathway. To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation. Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray. Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines. The major gene expression changes during irradiation are related to WNT signaling pathway. |
| Author | Yoon, Mee-Sun Oh, In-Jae Kim, Young-Chul Kim, Kyu-Sik Ban, Hee-Jung Nam, Taek-Keun Jeong, Jae-Uk Chung, Woong-Ki Ahn, Sung-Ja Choi, Chan Choi, Yoo-Duk Song, Joo-Young |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/25202076$$D View this record in MEDLINE/PubMed |
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| Copyright | Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved. |
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| Issue | 9 |
| Keywords | fractionated irradiation cDNA miroarrays radioresistance Non-small cell lung cancer |
| Language | English |
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| Snippet | To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
Lung cancer cell... BACKGROUNDTo identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.MATERIALS... Background: To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.... |
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| SubjectTerms | Cell Line, Tumor Cell Proliferation - radiation effects Cell Survival - radiation effects Cluster Analysis Dose Fractionation Dose-Response Relationship, Radiation Gene Expression Profiling Gene Expression Regulation, Neoplastic - radiation effects Humans Lung Neoplasms - genetics Lung Neoplasms - radiotherapy Mouse mammary tumor virus Radiation Tolerance - genetics Reproducibility of Results |
| Title | Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation |
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