Microarray analysis of gene expression in lung cancer cell lines treated by fractionated irradiation

• Published in: Anticancer research Vol. 34; no. 9; pp. 4939 - 4948
• Main Authors: Ahn, Sung-Ja, Choi, Chan, Choi, Yoo-Duk, Kim, Young-Chul, Kim, Kyu-Sik, Oh, In-Jae, Ban, Hee-Jung, Yoon, Mee-Sun, Nam, Taek-Keun, Jeong, Jae-Uk, Song, Joo-Young, Chung, Woong-Ki
• Format: Journal Article
• Language: English
• Published: Greece 01-09-2014
• Subjects: Cell Line, Tumor; Cell Proliferation - radiation effects; Cell Survival - radiation effects; Cluster Analysis; Dose Fractionation; Dose-Response Relationship, Radiation; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - radiation effects; Humans; Lung Neoplasms - genetics; Lung Neoplasms - radiotherapy; Mouse mammary tumor virus; Radiation Tolerance - genetics; Reproducibility of Results; fractionated irradiation; cDNA miroarrays; radioresistance; Non-small cell lung cancer
• ISSN: 0250-7005; 1791-7530

To identify differentially expressed genes between parent and radioresistant lung cancer cell lines established by fractionated irradiation.
Lung cancer cell lines (A549, NCI-H1650) were irradiated with several fractionation schemes. Clonogenic assays were used to identify radioresistant cell lines. We compared the gene expression profiles on a cDNA microarray.
Four established cell (A549-2G, A549-5G, H1650-2G and H1650-5G) were shown to be radioresistant (p≤0.05). Seventy-two genes were commonly altered in A549-G and 655 genes in H1650-G, compared to their parental cells. Genes in the wingless-type MMTV integration site family (WNT) signaling pathway were the ones most frequently altered in both A549-G and H1650-G cells. Those involved in inflammation; integrin, platelet-derived growth factor (PDGF), interleukin, transforming growth factor-beta (TGFB), epidermal growth factor receptor (EGFR) signaling, were commonly altered in radioresistant H1650 sublines.
The major gene expression changes during irradiation are related to WNT signaling pathway.