DNA extraction from fixed cytogenetic cell suspensions

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses,...

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Bibliographic Details
Published in:	Genetics and molecular research Vol. 6; no. 3; pp. 500 - 503
Main Authors:	Amorim, M R, Vargas, F R, Llerena, Jr, J C, Pombo-de-Oliveira, M S
Format:	Journal Article
Language:	English
Published:	Brazil 01-01-2007
Subjects:	Chromatography, High Pressure Liquid - methods Cytogenetics - methods DNA - analysis Exons Genome Humans Karyotyping Neoplasms - diagnosis Neoplasms - genetics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Analysis, DNA - methods
Online Access:	Get full text
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Summary:	We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1676-5680 1676-5680