Effect of potassium depletion on tubular morphology in gentamicin-induced acute renal failure in dogs

• Published in: Laboratory investigation Vol. 47; no. 6; p. 586
• Main Authors: Dobyan, D C, Cronin, R E, Bulger, R E
• Format: Journal Article
• Language: English
• Published: United States 01-01-1982
• Subjects: Acute Kidney Injury - chemically induced; Acute Kidney Injury - pathology; Animals; Creatinine - blood; Diet; Dogs; Gentamicins - adverse effects; Kidney Cortex - pathology; Kidney Cortex - ultrastructure; Kidney Tubules - pathology; Kidney Tubules - ultrastructure; Kidney Tubules, Proximal - pathology; Kidney Tubules, Proximal - ultrastructure; Male; Microscopy, Electron; Potassium - metabolism; Time Factors
• ISSN: 0023-6837

Potassium deprivation has recently been reported to potentiate the degree of functional impairment in a gentamicin-induced model of acute renal failure. The present study investigated the effects of two different states of potassium homeostasis on the development of cellular injury in the early stage of gentamicin nephrotoxicity in dogs. Gentamicin (15 mg. per kg. intramuscularly twice daily) was administered for 4 and 7 days to potassium-depleted or potassium-supplemented animals. The results show that potassium supplementation markedly lessens the severity of pathologic alterations induced by gentamicin. In both groups of animals, the S1 and S2 segments of the proximal tubule were the most consistently damaged regions of of the nephron. Potassium-supplemented dogs had a significantly higher number of normal proximal tubule cells than did the animals deprived of potassium and viewed 7 days after gentamicin treatment (77.3 versus 36.9 per cent; p less than 0.025). The degree of total injury to the proximal tubule was significantly higher in potassium-depleted animals than in those supplemented with potassium (59.9 versus 21.9 per cent; p less than 0.05). Only those dogs depleted of potassium prior to the administration of gentamicin had a markedly elevated plasma creatinine level of proximal tubular injury and functional impairment (r = 0.81; p less than 0.005). Potassium supplementation appears to lessen the extent of structural alterations seen in this model of gentamicin-induced acute renal failure in dogs.