Effects of physiological concentrations of steroid hormones and interleukin-11 on basal and stimulated production of interleukin-8 by human osteoblast-like cells with different functional profiles

• Published in: Clinical and experimental rheumatology Vol. 22; no. 1; pp. 79 - 84
• Main Authors: DOVIO, A, SARTORI, M. L, MASERA, R. G, PERETTI, L, PEROTTI, L, ANGELI, A
• Format: Journal Article
• Language: English
• Published: Pisa Clinical and Experimental Rheumatology 2004
• Subjects: Biological and medical sciences; Cell Line, Tumor; Diseases of the osteoarticular system; Dose-Response Relationship, Drug; Drug Combinations; Enzyme-Linked Immunosorbent Assay; Estradiol - pharmacology; Humans; Hydrocortisone - pharmacology; Inflammatory joint diseases; Interleukin-1 - pharmacology; Interleukin-11 - pharmacology; Interleukin-8 - metabolism; Medical sciences; Osteoblasts - drug effects; Osteoblasts - metabolism; Recombinant Proteins; Diseases of the osteoarticular system; Estrogen; Autoimmune disease; Inflammatory joint disease; Cortisol; Hydrocortisone; Estradiol; Profile; Production; Osteoblast; Cell; Interleukin 8; Human; Immunopathology; Corticosteroid; Steroid hormone; Rheumatology; Interleukin 11; Concentration; IL-8; IL-11; Chronic; Rheumatoid arthritis; Concentration effect; Adrenal hormone
• ISSN: 0392-856X; 1593-098X

IL-8 is a CXC chemokine involved in the pathogenesis of articular damage in rheumatoid arthritis. Local hyperproduction of IL-8 has been suggested to play a role in subchondral bone loss, since it suppresses osteoblast activity and promotes osteoclasts recruitment. Osteoblasts are a source of IL-8; its secretion is regulated by a number of hormones and cytokines. The aim of the present study was to evaluate the single and combined effects of physiological concentrations of cortisol, 17 beta-estradiol and IL-11 upon basal and IL-1 beta-inducible production of IL-8 in two human osteoblast-like cell lines, Saos-2 and MG-63.
Cells were incubated with cortisol (0.01 to 1 microM), 17 beta-estradiol (10 to 1000 pg/ml), IL-11 (1 to 100 ng/ml), in presence or absence of IL-1 beta (10 ng/ml), for 20 h. Combinations of 17 beta-estradiol and cortisol, and of IL-11 and cortisol, were also tested. After incubation, IL-8 levels in supernatants were measured by ELISA.
Cortisol dose-dependently inhibited spontaneous IL-8 secretion in both cell lines, although statistical significance was attained in the MG-63 cells only (P < 0.01); no effect of 17 beta-estradiol was apparent. With regard to IL-1 beta-inducible production, cortisol dose-dependently inhibited IL-8 release in both cell lines (P < 0.01); 17 beta-estradiol resulted in only a non-significant decrease in Saos-2, but not in MG-63 cells. 17 beta-estradiol did not alter the effects of cortisol in experiments involving co-incubation. IL-11 did not have any effect on spontaneous IL-8 release, but exerted a significant inhibitory effect on IL-1 beta-inducible release in MG-63 cells (P < 0.05); no additional effect was observed upon the degree of cortisol-dependent inhibition.
Cortisol is a potent physiological inhibitor of IL-8 production by osteoblast-like cells. The results of the present study support the use of exogenous supplemental glucocorticoids to prevent the deleterious effects of excess IL-8. The estrogenic milieu and local concentrations of IL-11 have little if any effect on the IL-8-dependent mechanisms of disease.