Purification of rickettsial cultures contaminated by mycoplasmas

An experimental biological model is proposed for purification of rickettsiae-infected cell cultures from mycoplasma contamination using intravenous mouse infection and subsequent passages on Vero cell monolayers. Mycoplasma-free rickettsial cultures were obtained in mouse brain or spleen suspension...

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Bibliographic Details
Published in:	Acta virologica (Anglickâa verze) Vol. 38; no. 4; p. 231
Main Authors:	Eremeeva, M E, Balayeva, N M, Raoult, D
Format:	Journal Article
Language:	English
Published:	Slovakia 01-08-1994
Subjects:	Animals Bacteriological Techniques Brain - microbiology Cells, Cultured Cercopithecus aethiops Male Mice Mycoplasma - isolation & purification Rickettsia - isolation & purification Vero Cells
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Summary:	An experimental biological model is proposed for purification of rickettsiae-infected cell cultures from mycoplasma contamination using intravenous mouse infection and subsequent passages on Vero cell monolayers. Mycoplasma-free rickettsial cultures were obtained in mouse brain or spleen suspension within 3 hrs or 3 days, respectively, after mouse inoculation. Ten strains of spotted fever group rickettsiae were purified from mycoplasmas by this procedure.
ISSN:	0001-723X