Autoradiographic localization of leptin binding in the choroid plexus of ob/ob and db/db mice

• Published in: Biochemical and biophysical research communications Vol. 219; no. 3; pp. 884 - 889
• Main Authors: Lynn, R B, Cao, G Y, Considine, R V, Hyde, T M, Caro, J F
• Format: Journal Article
• Language: English
• Published: United States 27-02-1996
• Subjects: Animals; Autoradiography; Binding Sites; Binding, Competitive; Brain - metabolism; Carrier Proteins - metabolism; Diabetes Mellitus - genetics; Diabetes Mellitus - metabolism; Female; Iodine Radioisotopes; Kinetics; Leptin; Mice; Mice, Inbred C57BL; Mice, Mutant Strains; Mice, Obese; Obesity - genetics; Obesity - metabolism; Proteins - metabolism; Receptors, Cell Surface; Receptors, Cytokine - metabolism; Receptors, Leptin; Species Specificity
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.1996.0328

The obese gene product, leptin, is synthesized in adipose tissue and is a circulating factor regulating body weight. To identify the location of leptin receptors in the brain we have performed an autoradiographic study of the binding of [(125)I]leptin to frozen sections of mouse brain. Dense specific binding of [(125)I]leptin was found only in the choroid plexus which is located in the dorsal part of the third ventricle and lateral ventricles. Specific binding of [(125)I]leptin was found the ob/ob and db/db mice. These findings further our understanding of the sites and mechanism of action of leptin on brain centers regulating body weight.