A molecular marker for the bovine ciliary neurotrophic factor receptor gene (CNTFR) maps to 8q21

A human cDNA encoding the ciliary neurotrophic receptor (CNTFR) was used to screen a bovine genomic library constructed in SuperCos1 (Stratagene) and consisting of more than 300 000 independent clones. A positive cosmid clone was subcloned. Sequencing of subclones yielded a 218-bp sequence, of which...

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Bibliographic Details
Published in:	Animal genetics Vol. 29; no. 1; p. 64
Main Authors:	Wigger, G, Masabanda, J, Stranzinger, G, Fries, R
Format:	Journal Article
Language:	English
Published:	England 01-02-1998
Subjects:	Animals Base Sequence Cattle - genetics Chromosome Mapping - veterinary Conserved Sequence Cosmids - chemistry Genetic Markers Humans In Situ Hybridization, Fluorescence - veterinary Mice Molecular Sequence Data Nerve Growth Factors - chemistry Nerve Growth Factors - genetics Receptor Protein-Tyrosine Kinases - chemistry Receptor Protein-Tyrosine Kinases - genetics Receptor, Ciliary Neurotrophic Factor Receptors, Nerve Growth Factor - chemistry Receptors, Nerve Growth Factor - genetics Receptors, Neurotransmitter - chemistry Receptors, Neurotransmitter - genetics Sequence Analysis, DNA - veterinary
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Summary:	A human cDNA encoding the ciliary neurotrophic receptor (CNTFR) was used to screen a bovine genomic library constructed in SuperCos1 (Stratagene) and consisting of more than 300 000 independent clones. A positive cosmid clone was subcloned. Sequencing of subclones yielded a 218-bp sequence, of which 167 bp were 90% similar to almost the entire exon 9 of the human CNTFR gene (EMBL accession number L38025). The EMBL accession number of the bovine sequence is Y11112.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0268-9146