Fibronectin peptide DRVPHSRNSIT and fibronectin receptor peptide DLYYLMDL arrest gastrulation of Rana pipiens

• Published in: Experientia Vol. 51; no. 11; p. 1097
• Main Authors: Wang, X, Lessman, C A, Taylor, D B, Gartner, T K
• Format: Journal Article
• Language: English
• Published: Switzerland 15-11-1995
• Subjects: Amino Acid Sequence; Animals; Dose-Response Relationship, Drug; Embryo, Nonmammalian - cytology; Embryo, Nonmammalian - drug effects; Embryo, Nonmammalian - physiology; Female; Fibronectins - chemistry; Fibronectins - pharmacology; Gastrula - drug effects; Gastrula - physiology; Male; Microinjections; Molecular Sequence Data; Peptide Fragments - administration & dosage; Peptide Fragments - pharmacology; Rana pipiens; Receptors, Fibronectin - chemistry; Structure-Activity Relationship
• ISSN: 0014-4754
• DOI: 10.1007/BF01946925

Gastrulation is characterized by dramatic cell migration which is thought to require the interaction of cell adhesion molecules with extracellular molecules. We have tested two novel peptides, a fibronectin peptide and a fibronectin receptor peptide, for their effects on gastrulation of the leopard frog Rana pipiens. The fibronectin peptide DRVPHSRNSIT corresponds to residues 1373-1383 of the cell-binding domain of fibronectin; the receptor peptide DLYYLMDL corresponds to residues 124-131 of beta 1 subunit of a variety of integrins including alpha 5 beta 1. Either of these peptides significantly inhibited gastrulation after being microinjected into mid-blastulae. These results indicate that these sequences may correspond to the ligand/receptor interaction sites of fibronectin and its receptor(s).