Stability of several LHRH antagonists against proteolytic enzymes and identification of degradation products by mass spectrometry

In this study stabilities of several LHRH antagonists against proteolytic enzymes are compared. For the enzymatic tests 15 proteases which differ in both substrate specificity and pH optimum were selected. The cyclic and two linear antagonists proved to be extraordinarily stable against the enzymes...

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Bibliographic Details
Published in:	Pharmazie Vol. 56; no. 1; p. 45
Main Authors:	Braun, K, Kuhl, P, Bernd, M, Kutscher, B
Format:	Journal Article
Language:	English
Published:	Germany 01-01-2001
Subjects:	Chromatography, Liquid Endopeptidases - chemistry Gonadotropin-Releasing Hormone - analogs & derivatives Gonadotropin-Releasing Hormone - antagonists & inhibitors Gonadotropin-Releasing Hormone - chemistry Hormone Antagonists - chemistry Hydrogen-Ion Concentration Mass Spectrometry Oligopeptides - chemistry Substrate Specificity
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Summary:	In this study stabilities of several LHRH antagonists against proteolytic enzymes are compared. For the enzymatic tests 15 proteases which differ in both substrate specificity and pH optimum were selected. The cyclic and two linear antagonists proved to be extraordinarily stable against the enzymes used over an incubation time of 50 h. Some degradation products were identified by high-performance liquid chromatography combined with mass spectrometry.
ISSN:	0031-7144