Alternative to polyacrylamide gels improves the electrophoretic mobility shift assay

In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGel 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simplicity and flexibility of agarose in the casting o...

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Bibliographic Details
Published in:	BioTechniques Vol. 18; no. 4; p. 704
Main Authors:	Vanek, P G, Fabian, S J, Fisher, C L, Chirikjian, J G, Collier, G B
Format:	Journal Article
Language:	English
Published:	England 01-04-1995
Subjects:	Base Sequence DNA-Binding Proteins - analysis Electrophoresis - methods Electrophoresis, Polyacrylamide Gel Gels HeLa Cells Humans Molecular Sequence Data Molecular Weight
Online Access:	Get more information
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Summary:	In this paper we outline a simplified protocol for the electrophoretic mobility shift assay utilizing TreviGel 500, a nontoxic alternative to polyacrylamide. The TreviGel 500 matrix combines the strength and resolution of polyacrylamide with the simplicity and flexibility of agarose in the casting of gels. Therefore, this method provides a simple, rapid and nontoxic alternative to current protocols for the investigation of protein: DNA interactions.
ISSN:	0736-6205