Lymphokine activated killer cells and interleukin-2 production in patients with cervical carcinoma

• Published in: Neoplasma Vol. 38; no. 5; pp. 493 - 499
• Main Authors: DE, A. K, JHAVER, K. G, KAMAT, M. R, NADKARNI, J. J
• Format: Journal Article
• Language: English
• Published: Bratislava Slovak Academic Press 1991
• Subjects: Adult; Aged; Antigens, Differentiation, T-Lymphocyte - biosynthesis; Biological and medical sciences; CD3 Complex; CD4 Antigens - biosynthesis; CD8 Antigens - biosynthesis; Cytotoxicity Tests, Immunologic; Cytotoxicity, Immunologic; Female; Gynecology. Andrology. Obstetrics; Humans; Immunophenotyping; Interleukin-2 - biosynthesis; Killer Cells, Lymphokine-Activated - immunology; Killer Cells, Lymphokine-Activated - metabolism; Medical sciences; Middle Aged; Phytohemagglutinins; Receptors, Antigen, T-Cell - biosynthesis; Uterine Cervical Neoplasms - metabolism; Human; Tumor; Cytotoxicity; Interleukin 2; LAK cell; Female genital diseases
• ISSN: 0028-2685

Lymphokine activated killer cells were generated from peripheral blood lymphocytes (PBL) in patients with cervical carcinoma. The cytotoxic potential of these cells was determined against three tumor cell lines (K-562, RAJI and MCF-7) using a short-term 51Cr-release assay. Both normals and patients showed comparable levels of LAK activity. Phenotypic analysis of cells having LAK activity showed a heterogeneous population. A significant increase in the expression of CD25 marker (IL-2R, Tac) of PBL population was observed after IL-2 culture. In addition, the indigenous ability of peripheral blood mononuclear cells to produce IL-2 in response to mitogen PHA was almost the same as observed in case of normal individuals.