A low-temperature-responsive translation elongation factor 1α from barley (Hordeum vulgare L.)

• Published in: Plant molecular biology Vol. 23; no. 1; pp. 221 - 225
• Main Authors: DUNN, M. A, MORRIS, A, JACK, P. L, HUGHES, M. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-10-1993
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Southern; DNA; Fundamental and applied biological sciences. Psychology; Genes. Genome; Hordeum - genetics; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Peptide Elongation Factor 1; Peptide Elongation Factors - chemistry; Peptide Elongation Factors - genetics; Plant Proteins - chemistry; Plant Proteins - genetics; Sequence Homology; Temperature; Monocotyledones; Hordeum vulgare; Nucleotide sequence; Arabidopsis; Sequence alignment; Homology; Chromosome 5; Chromosome 2; Cereal crop; Complementary DNA; Cruciferae; Gramineae; Dicotyledones; Angiospermae; Multigene family; Spermatophyta; Genome; Localization; Comparative study; Elongation factor EF1α; Aminoacid sequence; Low temperature
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00021434

A cDNA clone (pBLT63) encoding a protein synthesis elongation factor 1 alpha (EF-1 alpha) was isolated from a low-temperature winter barley shoot meristem library by differential screening. The nucleotide sequence of the coding region of the low-temperature-induced barley gene shows very high homology with two EF-1 alpha plant genes from tomato and Arabidopsis. The barley genome contains an EF-1 alpha gene family situated on the short arm of chromosome 2 and the long arm of chromosome 5.