Hemoglobin interference with urinary Bence Jones protein analysis on electrophoresis

Hemoglobin interference with urinary Bence Jones protein analysis on electrophoresis

Two cases are described which show that on urine protein electrophoresis a paraprotein owing to hemoglobin cannot be distinguished from a Bence Jones protein. A simple method is described for confirming the presence of hemoglobin: a densitometric scan of the electrophoretogram at 415 nm, in which he...

Full description

Saved in:
Bibliographic Details
Published in:Annals of clinical and laboratory science Vol. 26; no. 1; p. 71
Main Authors: Hobbs, G A, Levinson, S S
Format: Journal Article
Language:English
Published: United States 01-01-1996
Subjects:
Bence Jones Protein - urine
Electrophoresis
Hemoglobinuria - urine
Humans
Male
Middle Aged
Online Access:Get more information
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two cases are described which show that on urine protein electrophoresis a paraprotein owing to hemoglobin cannot be distinguished from a Bence Jones protein. A simple method is described for confirming the presence of hemoglobin: a densitometric scan of the electrophoretogram at 415 nm, in which hemoglobin absorbs in a band coincident with the restriction. Furthermore, it is shown that other serum proteins, elevated levels of bilirubin, and Bence Jones protein do not interfere with this detection system.
ISSN:0091-7370