Fine mapping of the sunflower resistance locus PlARG introduced from the wild species Helianthus argophyllus

• Published in: Theoretical and applied genetics Vol. 121; no. 8; pp. 1633 - 1644
• Main Authors: Wieckhorst, S., Bachlava, E., Dußle, C. M., Tang, S., Gao, W., Saski, C., Knapp, S. J., Schön, C.-C., Hahn, V., Bauer, E.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 2010; Springer
• Subjects: Agriculture; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetics of eukaryotes. Biological and molecular evolution; Helianthus; Helianthus annuus; Life Sciences; Methods, theories and miscellaneous; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plasmopara halstedii; Pteridophyta, spermatophyta; Vegetals; Bacterial Artificial Chromosome Clone; Linkage Group; Bacterial Artificial Chromosome; Simple Sequence Repeat Marker; Downy Mildew; Genetic mapping; Resistance; Introduced species; Dicotyledones; Helianthus; Angiospermae; Genetics; Spermatophyta; Agriculture; Compositae; Locus
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-010-1416-4

Downy mildew, caused by
Plasmopara halstedii
, is one of the most destructive diseases in cultivated sunflower (
Helianthus annuus
L.). The dominant resistance locus
Pl
ARG
originates from silverleaf sunflower (
H. argophyllus
Torrey and Gray) and confers resistance to all known races of
P. halstedii
. We mapped
Pl
ARG
on linkage group (LG) 1 of (cms)HA342 × ARG1575-2, a population consisting of 2,145 F
2
individuals. Further, we identified resistance gene candidates (RGCs) that cosegregated with
Pl
ARG
as well as closely linked flanking markers. Markers from the target region were mapped with higher resolution in NDBLOS
sel
 × KWS04, a population consisting of 2,780 F
2
individuals that does not segregate for
Pl
ARG
. A large-insert sunflower bacterial artificial chromosome (BAC) library was screened with overgo probes designed for markers RGC52 and RGC151, which cosegregated with
Pl
ARG
. Two RGC-containing BAC contigs were anchored to the
Pl
ARG
region on LG 1.