Sigma-1 Receptors Control Neuropathic Pain and Peripheral Neuroinflammation After Nerve Injury in Female Mice: A Transcriptomic Study

• Published in: Journal of neuroimmune pharmacology Vol. 19; no. 1; p. 46
• Main Authors: Ruiz-Cantero, M. Carmen, Entrena, José M., Artacho-Cordón, Antonia, Huerta, Miguel Á., Portillo-Salido, Enrique, Nieto, Francisco R., Baeyens, José M., Costigan, Michael, González-Cano, Rafael, Cobos, Enrique J.
• Format: Journal Article
• Language: English
• Published: New York Springer US 20-08-2024; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Biology; Female; Ganglia, Spinal - metabolism; Immunology; Mice; Mice, Inbred C57BL; Mice, Knockout; Neuralgia - metabolism; Neuroinflammatory Diseases - metabolism; Neurosciences; Pain; Peripheral Nerve Injuries - metabolism; Pharmacology/Toxicology; Receptors, sigma - antagonists & inhibitors; Receptors, sigma - genetics; Receptors, sigma - metabolism; Sigma-1 Receptor; Transcriptome; Virology; Neuroinflammation; Sigma-1 receptor; Artificial intelligence; T cell; Neuropathic pain; Macrophage
• ISSN: 1557-1890; 1557-1904; 1557-1904
• DOI: 10.1007/s11481-024-10144-8

The mechanisms for neuropathic pain amelioration by sigma-1 receptor inhibition are not fully understood. We studied genome-wide transcriptomic changes (RNAseq) in the dorsal root ganglia (DRG) from wild-type and sigma-1 receptor knockout mice prior to and following Spared Nerve Injury (SNI). In wildtype mice, most of the transcriptomic changes following SNI are related to the immune function or neurotransmission. Immune function transcripts contain cytokines and markers for immune cells, including macrophages/monocytes and CD4 + T cells. Many of these immune transcripts were attenuated by sigma-1 knockout in response to SNI. Consistent with this we found, using flow cytometry, that sigma-1 knockout mice showed a reduction in macrophage/monocyte recruitment as well as an absence of CD4 + T cell recruitment in the DRG after nerve injury. Sigma-1 knockout mice showed a reduction of neuropathic (mechanical and cold) allodynia and spontaneous pain-like responses (licking of the injured paw) which accompany the decreased peripheral neuroinflammatory response after nerve injury. Treatment with maraviroc (a CCR5 antagonist which preferentially inhibits CD4 + T cells in the periphery) of neuropathic wild-type mice only partially replicated the sigma-1 knockout phenotype, as it did not alter cold allodynia but attenuated spontaneous pain-like responses and mechanical hypersensitivity. Therefore, modulation of peripheral CD4 + T cell activity might contribute to the amelioration of spontaneous pain and neuropathic tactile allodynia seen in the sigma-1 receptor knockout mice, but not to the effect on cold allodynia. We conclude that sigma-1 receptor inhibition decreases DRG neuroinflammation which might partially explain its anti-neuropathic effect.
Graphical Abstract