Discordant plasma and cerebral spinal fluid cytokines/chemokines in relation to HIV-1-associated dementia

• Published in: Cellular and molecular biology (Noisy-le-Grand, France) Vol. 51 Suppl; p. OL745
• Main Authors: Killebrew, D A, Troelstrup, D, Valcour, V, Williams, A, Aguon, J, Sapalo, D, Shikuma, C, Ratto-Kim, S, Shiramizu, B
• Format: Journal Article
• Language: English
• Published: France 02-09-2005
• Subjects: Adult; Aged; Aged, 80 and over; AIDS Dementia Complex - blood; AIDS Dementia Complex - cerebrospinal fluid; AIDS Dementia Complex - immunology; Case-Control Studies; Chemokines - blood; Chemokines - cerebrospinal fluid; Enzyme-Linked Immunosorbent Assay; Female; Humans; Lipopolysaccharide Receptors; Macrophages - metabolism; Male; Middle Aged; Models, Biological; Predictive Value of Tests; Protein Array Analysis; Receptors, IgG; Subcellular Fractions
• ISSN: 1165-158X
• DOI: 10.1170/89

Monocytes and macrophages serve as HIV-1 reservoirs and may indirectly lead to HIV-1-associated dementia via neurotoxic cytokine/chemokine production. It remains unknown if peripheral monocytes and macrophages are responsible for the presence of circulating and cerebral spinal fluid cytokine/chemokine. The purpose of this evaluation was to determine the relationship between inflammatory and chemoattractant cytokine/chemokine in the periphery and the CNS among individuals with HIV-1-associated dementia and normal cognition. To accomplish this, we utilized specimens from the Hawaii Aging with HIV Cohort to assay plasma, cerebral spinal fluid, and cultured peripheral monocyte and macrophage supernatant cytokine/chemokines from individuals with HIV-1-associated dementia and normal cognition by ELISA, relative real-time PCR, and protein macroarrays. To further characterize the activated cells that may be responsible for HIV-1-associated dementia, inverse-PCR was used to identify sites of viral integration. Different mediators of inflammation, and chemoattraction from monocyte and macrophage supernatants, plasma, and cerebral spinal fluid were identified in HIV-1-associated dementia versus normal cognition. The data suggest unique pathways leading to cytokine/chemokine release in the periphery versus the brain region. This may have implications in delineating a cause and effect in HIV-1-associated dementia pathogenesis.