Detection of HIV-1 sequences in intestinal bacteria of HIV/AIDS patients

• Published in: Neuro-endocrinology letters Vol. 28; no. 5; p. 591
• Main Authors: Zajac, Vladimir, Stevurkova, Viola, Matelova, Lenka, Ujhazy, Eduard
• Format: Journal Article
• Language: English
• Published: Sweden 01-10-2007
• Subjects: Acquired Immunodeficiency Syndrome - drug therapy; Acquired Immunodeficiency Syndrome - virology; Anti-Retroviral Agents - therapeutic use; Base Sequence; Citrobacter freundii - genetics; DNA, Bacterial - analysis; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Enterobacter aerogenes - genetics; Enterobacteriaceae - genetics; Genes, Bacterial - genetics; Genes, env - genetics; Genes, gag - genetics; Genes, pol - genetics; Genes, Viral - genetics; HIV Infections - drug therapy; HIV Infections - virology; HIV-1 - genetics; Homosexuality; Humans; Intestines - microbiology; Intestines - virology; Male; Molecular Sequence Data; Proteus mirabilis - genetics; Reference Values; RNA, Viral - isolation & purification; Staphylococcus - genetics
• ISSN: 0172-780X

Bacterial DNA isolated from the intestinal tract of 11 American and 30 Slovak HIV/AIDS patients were analyzed by colony and dot blot hybridization assay for HIV-1 specific sequences. Secondly, PCR using primers specific for the HIV-1 gag, pol and env genes for detection of HIV-1 sequences in these DNA were performed.
Intestinal bacteria DNA of HIV/AIDS patients hybridized in colony and dot blot hybridization assay for HIV-1 specific sequences. PCR products synthesized on specific primers of HIV-1 gag (115 bp), env (142 bp), pol-env (1484) genes were found to be for more than 90% homologous to the corresponding sequence in HIV-1.
Intestinal bacteria of HIV/AIDS patients are bearing sequences for more than 90% identical with those of HIV-1.