Dna damage in peripheral blood nuclear cells assessed by comet assay from individuals submitted to scintigraphic examinations

• Published in: Cellular and molecular biology (Noisy-le-Grand, France) Vol. 48; no. 7; p. 789
• Main Authors: Dantas, F J S, de Mattos, J C P, Moraes, M O, Boasquevisques, E, Rodrigues, M P, Lage, C A S, Cabral-Neto, J B, Leitão, A C, Bernardo-Filho, M, Bezerra, R J A C, Carvalho, J J, Caldeira-de-Araujo, A
• Format: Journal Article
• Language: English
• Published: France 01-11-2002
• Subjects: Apoptosis - radiation effects; Comet Assay; DNA Damage; DNA Repair; Humans; Leukocytes - metabolism; Leukocytes - pathology; Leukocytes - radiation effects; Radiopharmaceuticals - adverse effects; Technetium - adverse effects; Tin Compounds - adverse effects
• ISSN: 0145-5680

Stannous chloride (SnCl2) is employed as a reducing agent to obtain Technetium-99m-labelled radiophamaceuticals in nuclear medicine kits, being injected endovenously in humans. Toxic effects of these kits were not studied, thus making it important to evaluate their impact in humans. In this study, the toxic effects were evaluated from peripheral blood nuclear cells (PBNC) from patients who received radiopharmaceuticals obtained using such kits. The analyses included results performed by comet assay. DNA damage was visualized in PBNC samples collected within a time up to 2 hr, and 24 hr after radiopharmaceutical injection in the patients. Initially we observed an increase of comet signals, which subsequently were reduced to zero after 24 hr. The diminishing of comet amounts probably is associated with DNA repair of damaged cells or with the elimination by apoptosis of cells whose DNA are not repaired.