Antioxidant status and levels of different vitamins determined by high performance liquid chromatography in diabetic subjects with multiple complications

• Published in: General physiology and biophysics Vol. 22; no. 1; pp. 15 - 27
• Main Authors: Merzouk, S, Hichami, A, Madani, S, Merzouk, H, Berrouiguet, A Y, Prost, J, Moutairou, K, Chabane-Sari, N, Khan, N A
• Format: Journal Article
• Language: English
• Published: Slovakia 01-03-2003
• Subjects: Adult; Antioxidants - analysis; Antioxidants - metabolism; Ascorbic Acid - blood; Ascorbic Acid - metabolism; Catalase - blood; Catalase - metabolism; Chromatography, High Pressure Liquid - methods; Coronary Artery Disease - blood; Coronary Artery Disease - etiology; Coronary Artery Disease - metabolism; Diabetes Complications; Diabetes Mellitus - blood; Diabetes Mellitus - classification; Diabetes Mellitus - metabolism; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 1 - classification; Diabetes Mellitus, Type 1 - complications; Diabetes Mellitus, Type 1 - metabolism; Diabetes Mellitus, Type 2 - blood; Diabetes Mellitus, Type 2 - classification; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - metabolism; Erythrocytes - enzymology; Female; Glutathione Peroxidase - blood; Glutathione Peroxidase - metabolism; Glutathione Reductase - blood; Glutathione Reductase - metabolism; Humans; Hypertension - blood; Hypertension - etiology; Hypertension - metabolism; Male; Middle Aged; Renal Insufficiency - blood; Renal Insufficiency - etiology; Renal Insufficiency - metabolism; Superoxide Dismutase - blood; Superoxide Dismutase - metabolism; Vitamin A - blood; Vitamin A - metabolism; Vitamin E - blood; Vitamin E - metabolism; Vitamins - blood; Vitamins - metabolism
• ISSN: 0231-5882

Plasma vitamin A, C and E levels and erythrocyte antioxidant enzyme activities were investigated in type I and type II diabetic subjects with and without complications, i.e., hypertension, coronary artery disease and renal failure. Reverse phase HPLC was used to quantify vitamin A and E levels. We observed that the vitamin C levels were not significantly different between control and diabetic subjects. However, vitamin A and E levels were significantly lower in type I and type II diabetic subjects compared to controls. Superoxide dismutase (SOD) activity was significantly lower in type II, but not in type I, diabetic patients compared to controls. Interestingly, glutathione reductase and peroxidase activities were diminished in type I, but not in type II, diabetic subjects as compared to controls. Catalase activity was lower in both types of diabetic patients in comparison with their respective controls. Altogether these results suggest that diabetes mellitus may be associated with altered antioxidant status regardless to various complications.