Genetic and physical delineation of the region of the mouse deafness mutation shaker-2

• Published in: Biochemical and biophysical research communications Vol. 234; no. 1; pp. 107 - 110
• Main Authors: Wakabayashi, Y, Kikkawa, Y, Matsumoto, Y, Shinbo, T, Kosugi, S, Chou, D, Furuya, M, Jishage, K, Noda, T, Yonekawa, H, Kominami, R
• Format: Journal Article
• Language: English
• Published: United States 08-05-1997
• Subjects: Animals; Chromosome Mapping; Chromosome Walking; Chromosomes, Artificial, Yeast; Cloning, Molecular; Crosses, Genetic; Deafness - genetics; Genes, Recessive; Genetic Linkage - genetics; Haplotypes - genetics; Mice; Mice, Mutant Strains; Mutation; Polymerase Chain Reaction
• ISSN: 0006-291X
• DOI: 10.1006/bbrc.1997.6595

A total of 951 backcross progeny have been obtained from a backcross segregating for the mouse deafness mutation, shaker-2(sh-2). Linkage analysis provides a detailed genetic map in the vicinity of sh-2 which comprises 40 backcross mice identified as recombinant within a 4 cM region. This allows construction of a contig consisting of 21 BAC clones across an approximately 700-kb region of sh-2. This covers the entire nonrecombinant region of sh-2 and is therefore useful to facilitate the identification of genes in the sh-2 region.