Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues

An immunohistochemical assay was used to assess expression of ras p21 and myc p62 oncogene products in human hepatocellular carcinoma (HCC) and non-neoplastic liver tissues. The monoclonal antibodies Y13 259 and Myc1-9E10, specific for ras p21 and myc p62 oncoproteins, were employed on paraffin-embe...

Full description

Saved in:

Bibliographic Details
Published in:	Anticancer research Vol. 9; no. 3; p. 715
Main Authors:	Tiniakos, D, Spandidos, D A, Kakkanas, A, Pintzas, A, Pollice, L, Tiniakos, G
Format:	Journal Article
Language:	English
Published:	Greece 01-05-1989
Subjects:	Carcinoma, Hepatocellular - analysis Carcinoma, Hepatocellular - genetics Humans Immunohistochemistry Liver - analysis Liver Neoplasms - analysis Liver Neoplasms - genetics Proto-Oncogene Proteins - analysis Proto-Oncogene Proteins c-myc Proto-Oncogene Proteins p21(ras)
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	An immunohistochemical assay was used to assess expression of ras p21 and myc p62 oncogene products in human hepatocellular carcinoma (HCC) and non-neoplastic liver tissues. The monoclonal antibodies Y13 259 and Myc1-9E10, specific for ras p21 and myc p62 oncoproteins, were employed on paraffin-embedded sections. Most HCCs showed enhanced ras p21 and myc p62 expression, as indicated by staining intensity. Cirrhotic livers revealed increased myc p62 and occasionally increased ras p21 expression. HBsAg+ hepatocytes showed intense immunostaining for ras p21. Fibrotic, cholestatic, fetal and normal adult liver did not present enhancement of oncoprotein production. We suggest that combined over-expression of ras and myc oncoproteins may be important for the malignant phenotypic alteration in human HCC.
ISSN:	0250-7005