Immunoelectron microscopic and immunochemical demonstrations of serum proteins in the alveolar lining material of the rat lung

By means of antibodies labeled with horseradish peroxidase, autologous albumin and IgG were visualized in electron microscopy on the alveolar epithelial surface of the rat lung, as a continuous cell coat, as free granular deposits, or associated with tubular myelin figures. These 2 proteins were det...

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Bibliographic Details
Published in:	The American review of respiratory disease Vol. 113; no. 2; p. 109
Main Authors:	Bignon, J, Jaurand, M C, Pinchon, M C, Sapin, C, Warnet, J M
Format:	Journal Article
Language:	English
Published:	United States 01-02-1976
Subjects:	Animals Blood Proteins - analysis Horseradish Peroxidase Immunochemistry Immunoglobulin G - analysis Microscopy, Electron Pulmonary Alveoli - analysis Pulmonary Surfactants - analysis Rabbits - immunology Rats Serum Albumin - analysis Tubulin
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Summary:	By means of antibodies labeled with horseradish peroxidase, autologous albumin and IgG were visualized in electron microscopy on the alveolar epithelial surface of the rat lung, as a continuous cell coat, as free granular deposits, or associated with tubular myelin figures. These 2 proteins were detected by immunochemistry in alveolar washing. After ultracentrifugation of alveolar washing, they were still found in the phospholipid-rich precipitate fraction by immunization of rabbits and by immunoelectron microscopy. Thus, albumin and IgG appeared as normal alveolar components in the rat lung; fibrinogen was not found under normal conditions.
ISSN:	0003-0805