Pharmacology of Ca2+ release from red beet microsomes suggests the presence of ryanodine receptor homologs in higher plants

• Published in: FEBS letters Vol. 395; no. 1; pp. 39 - 42
• Main Authors: Muir, Shelagh R., Sanders, Dale
• Format: Journal Article
• Language: English
• Published: England 14-10-1996
• Subjects: 2-[N-morpholino]ethanesulphonic acid; Adenosine Diphosphate Ribose - analogs & derivatives; Adenosine Diphosphate Ribose - metabolism; Beta vulgaris; cADPR; Caffeine - pharmacology; Calcium; Calcium - metabolism; Calcium Channel Agonists - pharmacology; Calcium Channel Blockers - pharmacology; Calcium Channels - chemistry; carbonyl cyanide p-trifluoromethoxyphenylhydrazone; Cyclic ADP-Ribose; FCCP; Heparin - pharmacology; inositol 1,4,5-trisphosphate; Inositol 1,4,5-Trisphosphate - pharmacology; InsP3; Ion Channel Gating - drug effects; Mes; Microsome; Microsomes - metabolism; Muscle Proteins - chemistry; phenylmethylsulphonyl fluoride; PMSF; Procaine - pharmacology; Ryanodine; Ryanodine - pharmacology; Ryanodine Receptor Calcium Release Channel; Vegetables - physiology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(96)01000-9

Cyclic ADP‐ribose (cADPR) is known to release Ca2+ from plant vacuoles, implying that this NAD+ metabolite may possess a second messenger role in plants. The degree to which the plant cADPR‐gated Ca2+ release mechanism resembles cADPR action in animals has been evaluated. cADPR‐elicited Ca2+ release from red beet microsomes was inhibited by 1 mM procaine but insensitive to heparin. Furthermore, pre‐release of Ca2+ from red beet vesicles by either 5 mM caffeine or micromolar levels of ryanodine precluded further Ca2+ mobilisation by cADPR. Thus, this study argues strongly for conservation between the plant and animal cADPR‐elicited Ca2+ release mechanisms.