The stereochemical resolution of enantiomeric free and derivatized amino acids using an HPLC chiral stationary phase based on immobilized alpha-chymotrypsin: chiral separation due to solute structure or enzyme activity

• Published in: Chirality (New York, N.Y.) Vol. 1; no. 1; p. 38
• Main Authors: Jadaud, P, Thelohan, S, Schonbaum, G R, Wainer, I W
• Format: Journal Article
• Language: English
• Published: United States 1989
• Subjects: Amino Acids - isolation & purification; Chromatography, High Pressure Liquid; Chymotrypsin; Enzymes, Immobilized; Solutions; Stereoisomerism
• ISSN: 0899-0042
• DOI: 10.1002/chir.530010109

The stereochemical separation of free and derivatized amino acids on active alpha-chymotrypsin bonded to silica is governed by two mechanisms based on the structure of the solutes or on the enzymatic activity of the enzyme. The deactivation of the hydrolytically active site of the enzyme demonstrated that a significant portion of the retention on this support is due to hydrophobic interactions at other sites. These sites appear to be stereoselective for the ester derivatives of amino acids but not for the other studied solutes.