Methods for in vivo and in vitro analysis of innate immune responses to Helicobacter pylori infection

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 921; p. 209
• Main Authors: Patel, Milan K, Ryan, Glennice N, Cerny, Anna M, Kurt-Jones, Evelyn A
• Format: Journal Article
• Language: English
• Published: United States 2012
• Subjects: Animals; Cell Culture Techniques; Cell Separation; Cytokines - secretion; Dissection; Enzyme-Linked Immunosorbent Assay; HEK293 Cells; Helicobacter Infections - immunology; Helicobacter Infections - microbiology; Helicobacter pylori - immunology; Helicobacter pylori - pathogenicity; Humans; Immunity, Innate; Luciferases - genetics; Macrophages - cytology; Macrophages - immunology; Macrophages - secretion; Mice; Monocytes - cytology; Monocytes - immunology; Monocytes - secretion; Species Specificity; Stomach - microbiology; Stomach - pathology; Transfection
• ISSN: 1940-6029
• DOI: 10.1007/978-1-62703-5-2_24

It is estimated that half of the world's population is infected by Helicobacter pylori (H. pylori) (Polk and Peek, Nat Rev Cancer 10:403-414, 2010; Peek et al., Physiol Rev 90:831-858, 2010). Following infection, H. pylori induces a chronic innate immune response that is thought to contribute to gastric complications. Due to the widespread prevalence of H. pylori, it is important to study the innate immune responses that result from the infection. A variety of in vitro and in vivo techniques have been developed by our laboratory to study this immune response (Fox et al., Am J Pathol 171:1520-1528, 2007; Kurt-Jones et al., Infect Immun 75:471-480, 2007; Kurt-Jones et al., J Endotoxin Res 10:419-424, 2004). These methods are described here.