High efficiency indirect shoot regeneration and hypericin content in embryogenic callus of Hypericum triquetrifolium Turra

• Published in: African journal of biotechnology Vol. 9; no. 15; pp. 2229 - 2233
• Main Authors: Oluk, E A, Orhan, S, Karakas, Oe, Cakir, A, Goenuez, A
• Format: Journal Article
• Language: English
• Published: 12-04-2010
• Subjects: Hypericum
• ISSN: 1684-5315; 1684-5315

A method to induce indirect regeneration from Hypericum triquetrifolium Turra was described in the present study. Callus was induced from cotyledon explants of 35 days old aseptic seedlings on semi-solid MS supplemented with IAA (0.5 mg.L super(-1)) combined with BAP (2 mg.L super(-1)). Meristemoids developed on the surface of callus by decreasing of the cytokinins and plantlet regeneration with 100% frequency through these embryogenic calli occured in semi-solid medium when the PGRs were removed completely. Embryogenic calli obtained during the experiments were analyzed for their hypericin content. And they were found to produce hypericin as 48 kg/g DM. Regenerated plantlets were rooted in MS containing 1 mg/L IAA. The highest percentages (94%) of survival of transferred plantlets to free-living circumstances were limited when they were acclimatized in sand: peat: perlite (1:1:1; v/v/v) mixture.