Formation of Extracellular Enzyme Systems during the Growth of Geotrichum candidum 3C on Cell Walls Isolated from Cereal Grain Coats

• Published in: Applied biochemistry and microbiology Vol. 37; no. 5; pp. 480 - 482
• Main Authors: Rodionova, NA, Dubovaya, N V, Martinovich, LI, Bezborodov, A M
• Format: Journal Article
• Language: English
• Published: 2001
• Subjects: Geotrichum candidum; hemicellulase; Triticum
• ISSN: 0003-6838
• DOI: 10.1023/A:1010241923100

The activities of extracellular systems of hemicellulases, pectinases, and cellulases was studied during a 72-h cultivation of Geotrichum candidum 3C. The culture was grown on a medium containing 3% cell walls isolated from wheat grain coats, which served as the sole carbon source. Enzymes catalyzing the degradation of pectin substances (beet pectin, alpha -L-arabinan, and 1,4- beta -D-galactan), as well as beta -D-galactosidase and alpha -L-arabinofuranosidase involved in their hydrolysis, were formed first (4 h after the beginning of cultivation). Enzymes hydrolyzing 4-O-methyl- alpha -D-glucurono- beta -D-xylan and sodium carboxymethyl xylan were also found in the culture liquid after 4 h of fungal growth. The contents of pectin-degrading and xylanolytic enzymes reached their maximum levels after 52-56 and 72 h of growth, respectively. Cellulolytic enzymes were detected after 8-28 h of cultivation. Enzymes degrading alpha -D-galacto- beta -D-mannan were found 24 h after the beginning of growth; their content was maximum after 72 h of cultivation.