The effect of chlorpromazine and dibucaine on phospholipid metabolism in the frog sartorius muscle

The effect of chlorpromazine and dibucaine on phospholipid metabolism in the frog sartorius muscle

The effect of cationic amphiphilic drugs, chlorpromazine and dibucaine, on phospholipid metabolism in the frog sartorius muscle was studied at concentrations affecting excitation-contraction coupling. Labelling patterns of phospholipids using 32P orthophosphate were determined. The drugs at a concen...

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Bibliographic Details
Published in:Physiologia Bohemoslovaca Vol. 33; no. 1; p. 59
Main Authors: Novotný, I, Saleh, F, Novotná, R
Format: Journal Article
Language:English
Published: Czech Republic 1984
Subjects:
Animals
Calcium - pharmacology
Cell Membrane Permeability - drug effects
Chlorpromazine - pharmacology
Dibucaine - pharmacology
In Vitro Techniques
Muscles - metabolism
Phosphatidylinositols - metabolism
Phosphatidylserines - metabolism
Phospholipids - metabolism
Rana temporaria
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Summary:The effect of cationic amphiphilic drugs, chlorpromazine and dibucaine, on phospholipid metabolism in the frog sartorius muscle was studied at concentrations affecting excitation-contraction coupling. Labelling patterns of phospholipids using 32P orthophosphate were determined. The drugs at a concentration of 3.10(-4) mol/l inhibited the synthesis of phosphatidylcholine and phosphatidylethanolamine and stimulated the synthesis of phosphatidylinositol and phosphatidylserine. At the 3.10(-3) mol/l concentration the drugs blocked the synthesis of all phospholipids without appreciably affecting their degradation. The effect of cationic amphiphilic drugs was independent of the presence of Ca2+ or K+ ions in the media, however, basal labelling of phospholipids was affected by withdrawal of Ca2+ or K+ ions.
ISSN:0369-9463