Measuring Urease and Phospholipase Secretion in Cryptococcus neoformans

Urease and phospholipase are enzymes that are important virulence factors for Cryptococcus neoformans. These are two of the most studied enzymes involved in how C. neoformans breaches the blood-brain barrier. Additionally, phospholipase secretion also supports dissemination from the lungs. This chap...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2775; p. 269
Main Authors:	Hoffman, Hunter J, McClelland, Erin E
Format:	Journal Article
Language:	English
Published:	United States 2024
Subjects:	Cryptococcosis - microbiology Cryptococcus neoformans - enzymology Cryptococcus neoformans - pathogenicity Fungal Proteins - metabolism Humans Phospholipases - metabolism Urease - metabolism Virulence Virulence Factors - metabolism Cryptococcus Phospholipase Urease Virulence
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Summary:	Urease and phospholipase are enzymes that are important virulence factors for Cryptococcus neoformans. These are two of the most studied enzymes involved in how C. neoformans breaches the blood-brain barrier. Additionally, phospholipase secretion also supports dissemination from the lungs. This chapter describes the methods used to measure the secretion of these enzymes, which may be used to characterize strain invasiveness and virulence.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-3722-7_18