Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa Fluor 488 and PEG Immunohistochemistry

Evaluation of Nanomedicine Tissue Distribution and Stability by Alexa Fluor 488 and PEG Immunohistochemistry

A primary issue with nanomedicine biological evaluation is determination of nanoparticle carrier tissue distribution and stability. Here we present a method to evaluate nanomedicine distribution in tissues that is applicable to most nanomedicine constructs. This method utilizes immunohistochemical (...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 2789; p. 313
Main Authors: Ware, Teagan D, Butcher, Donna, Edmondson, Elijah, Stern, Stephan T
Format: Journal Article
Language:English
Published: United States 2024
Subjects:
Fluoresceins
Fluorescent Dyes
Immunohistochemistry
Nanomedicine
Polyethylene Glycols
Sulfonic Acids
Tissue Distribution
Immunohistochemistry
PEG
Biodistribution
Nanomedicine
Alexa488
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Summary:A primary issue with nanomedicine biological evaluation is determination of nanoparticle carrier tissue distribution and stability. Here we present a method to evaluate nanomedicine distribution in tissues that is applicable to most nanomedicine constructs. This method utilizes immunohistochemical (IHC) analysis of an Alexa Fluor 488-tag and/or polyethylene glycol (PEG), a very common nanomedicine component, for tissue localization. Using specific Alexa Fluor 488- and/or PEG antibody-based IHC staining procedures allows evaluation of high-resolution nanoparticle tissue distribution, nanoparticle tissue stability, and also allows correlation of distribution with morphological changes. This protocol outlines the methods to follow to ensure proper tissue collection and optimized immunohistochemical staining of Alexa Fluor 488-tag and PEG in tissues.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3786-9_28