Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection

Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensi...

Full description

Saved in:

Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 2788; p. 67
Main Authors:	Cruz-Rubio, José Manuel, Loeppert, Renate, Praznik, Werner
Format:	Journal Article
Language:	English
Published:	United States 2024
Subjects:	Antipyrine - analogs & derivatives Antipyrine - chemistry Chromatography, High Pressure Liquid - methods Chromatography, Reverse-Phase - methods Edaravone - chemistry Monosaccharides - analysis Monosaccharides - chemistry Spectrophotometry, Ultraviolet - methods 1-Phenyl-3-methyl-5-pyrazolone Monosaccharide derivatization Superficially porous columns PMP Monosaccharide chromophores Chromatography Carbohydrate analysis
Online Access:	Get more information
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a superficially porous C18 reverse-phase column, using common liquid chromatography equipment, results in short analysis times (under 20 min) and high sensitivity (limit of quantitation 1 nmol). This method allows for complex monosaccharide mixtures to be separated and quantified using a reasonably simple and safe derivatization procedure.
ISSN:	1940-6029
DOI:	10.1007/978-1-0716-3782-1_5