Preparation and characterization of specific monoclonal antibodies against mercury ions

• Published in: Shengwu gongcheng xuebao Vol. 26; no. 6; p. 753
• Main Authors: Zhao, Li, Wang, Fenglong, Yang, Hui, Li, Peng, Liu, Manxing, Li, Xia
• Format: Journal Article
• Language: Chinese
• Published: China 01-06-2010
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Chelating Agents - chemistry; Environmental Pollutants - analysis; Environmental Pollutants - immunology; Female; Hybridomas - metabolism; Immunoassay - methods; Mercury - analysis; Mercury - immunology; Mice; Mice, Inbred BALB C
• ISSN: 1000-3061

The environmental pollution by heavy metals such as mercury, cadmium and lead has become a worldwide public health hazard. To rapidly and inexpensively monitor environmental heavy metals is a prerequisite for minimizing human and animal exposure. The development of immunoassays to detect mercury ion residues has been a promising trend with the advantage of rapid and cheap operation. We reported the isolation and characterization of mercury-specific monoclonal antibodies. Because Hg2+ ions are too small to elicit an immune response, the metal was coupled to protein carrier (keyhole limpet, KLH) using a chelator (diethylenetriamine pentaacetic acid, DTPA). After the synthesis of antigen and characterization, monoclonal antibodies against mercury ions were generated by immunizing BALB/c mice with mercury conjugated antigen (Hg-DTPA-KLH). The stable hybridoma cell lines were produced by fusion of murine splenocytes and SP2/0 myeloma cells. The hybridoma cells were subcloned by the limiting dilution and screened b