Ets-related protein E1A-F can activate three different matrix metalloproteinase gene promoters

An Ets-related E1A-F has been characterized as an enhancer-binding protein for the adenovirus E1A gene. Here we show, in transient expression assays, that E1A-F can activate three different subclasses of the matrix metalloproteinase gene promoters. Expressions of the chloramphenicol acetyltransferas...

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Bibliographic Details
Published in:	Oncogene Vol. 10; no. 7; p. 1461
Main Authors:	Higashino, F, Yoshida, K, Noumi, T, Seiki, M, Fujinaga, K
Format:	Journal Article
Language:	English
Published:	England 06-04-1995
Subjects:	Adenovirus E1A Proteins - physiology DNA-Binding Proteins - physiology Gene Expression Regulation, Neoplastic Humans In Vitro Techniques Matrix Metalloproteinase 3 Metalloendopeptidases - genetics Osteosarcoma Promoter Regions, Genetic Proto-Oncogene Proteins - physiology RNA, Messenger - genetics Transcription Factors - physiology Transcription, Genetic Tumor Cells, Cultured
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Summary:	An Ets-related E1A-F has been characterized as an enhancer-binding protein for the adenovirus E1A gene. Here we show, in transient expression assays, that E1A-F can activate three different subclasses of the matrix metalloproteinase gene promoters. Expressions of the chloramphenicol acetyltransferase (CAT) reporter gene under the control of stromelysin, type I collagenase and 92 kD type IV collagenase promoters were increased approximately 10- to 20-fold by co-transfection with the E1A-F expression vector. Activation levels were as much high as those obtained by exogenous expression of AP-1 transcription factor. These results suggest that E1A-F positively regulates transcriptions from matrix metalloproteinase genes that are associated with invasion and metastasis of tumor cells.
ISSN:	0950-9232