Wuchereria bancrofti : Detection of microfilariae in asymptomatic microfilaremic individuals with Setaria digitata antigens

• Published in: Southeast Asian journal of tropical medicine and public health Vol. 32; no. 2; pp. 230 - 234
• Main Authors: WICKREMANAYAKE, M. N, EKANAYAKE, Sriyani, KARUNANAYAKE, Eric H
• Format: Journal Article
• Language: English
• Published: Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 01-06-2001
• Subjects: Animals; Antigens, Helminth; Biological and medical sciences; Blotting, Western; Diseases caused by nematodes; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Filariases; Filariasis - diagnosis; Helminthic diseases; Humans; Infectious diseases; Lymphatic filariases; Medical sciences; Parasitic diseases; Setaria Nematode - immunology; Sri Lanka; Tropical medicine; Wuchereria bancrofti - isolation & purification; Sri Lanka; Parasitological investigation; Human; Lymphatic system; Microfilaria; Wuchereria bancrofti; Parasitosis; Helminthiasis; Blood; Infection; Nematode disease; Helmintha; Nemathelminthia; Diagnosis; Filariosis; Invertebrata; Nematoda
• ISSN: 0125-1562

A dot-ELISA for detection of microfilariae of Wuchereria bancrofti in an endemic area was developed. This test can differentiate the endemic normals from the microfilaraemic asymptomatic individuals. Antigens of molecular weight 130 and 52 kDa of the cattle filaria worm Setaria digitata were used for this test. It was observed that these two antigens were also present in the serum of asymptomatic microfilaraemic individuals.